Browsing by Author "T.Anthoney Swamy"

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    Antibacterial activity of Methanolic extracts of Cola nitida Seeds on Selected Pathogenic Organisms
    (International Journal of Current Microbiology and Applied Sciences (IJCMAS), 2014) Jackie K. Obey; T.Anthoney Swamy
    Cola nitida has been used in folk medicine and is a member of the family Steculicca. The aim of the current study was to investigate the antibacterial activity of Cola nitida. Antibacterial screening was done using agar well diffusion method against Bacillus cereus, Serratia marcescens, Staphyloccus epidermidis, Proteus vulgaris and Salmonella typhi. The results of antibacterial activity of red Cola nitida showed a zone of inhibition of 23.67±0.882 on Bacillus cereus, 22.67±1.452 on Serratia marcescens, 24.33±0.667 on Staphyloccus epidermidis and 13.00±0.577 on Proteus vulgaris. The penicillin control showed large zones of inhibition except for B. cereus and DMSO did not show any zones of inhibition. The antibacterial property shown by the plant extracts is an evidence of the ethnomedicinal uses of the plants. Incorporation of active compounds isolated from the methanol extract of Cola nitida into conventional drug preparations can also tackle the challenges posed by drug resistant microorganisms.
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    In vitro evaluation of antibacterial activity of infused Cola nitida seeds
    (Int.J.Curr.Microbiol.App.Sci, 2014) Jackie K. Obey; T.Anthoney Swamy
    The aim of the current work is to investigate the antibacterial activity of infused Cola nitida seeds. Antibacterial screening was done using agar well diffusion method against Bacillus cereus, Serratia marcescens, Staphyloccus epidermidis, Proteus vulgaris and Salmonella typhi. The extract inhibited the growth of Bacillus cereus with a zone of inhibition of 14.33±0.882, Serratia marcescens 26.67±1.202 and Staphylococcus epidermidis 12.33±0.577. The penicillin control showed large zones of inhibition and DMSO did not show any zones of inhibition. Analysis of variance (ANOVA) performed on the data showed that there were significant differences in the zone of inhibition formed by the extract and antibiotic control (p<0.0001). A Tukey s multiple comparison tests also revealed several differences in the zones of inhibition of the extract between organisms and between all the organisms and their controls. Few pairwise comparisons showed that the zones did not differ significantly between organisms. The present study has proved that it is possible to control the growth of some pathogenic microorganisms using the infused extract of Cola nitida. The infused extracts from the plant can therefore be considered by pharmaceutical industries in production of cheap, affordable and available drugs for the cure of infections caused by these organisms in situations where expensive conventional drugs are unavailable.
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    Preliminary phytochemical and in vitro control of selected pathogenic organisms by ethanolic extract of Garcinia kola seeds
    (Int.J.Curr.Microbiol.App.Sci, 2014) Obey Jackie; T.Anthoney Swamy; Ngule Chrispus Mutuku
    Garcinia kola is cultivated for its economic importance. The plant is mainly used as a medicinal herb in West Africa. Although it is bitter the plant is used as a snack and a stimulant due to the high content of caffeine in the seeds of the plant. The current study was done to investigate the antibacterial activity of the plant seeds and to analyse the presence of important pharmaceutical compounds. From the study the plant seeds were found to contain tannins, saponins, flavonoids, terpenoids, glycosides and alkaloids but phenols and steroids were found to be absent in the plant seeds. Among all the organisms tested with the ethanol extract of G. kola, only B. cereus with an inhibition zone of 10.17±0.477 and E. coli with an inhibition zone of 12.83±0.833 were inhibited. All other organisms were not inhibited. The penicillin control showed large zones of inhibition and DMSO did not show any zones of inhibition. The data obtained in this research is a scientific justification of the plants traditional use in the treatment of various stomach problems. From this research it is worthy to recommend the plant seeds for the treatment of diarrhea caused by E.coli and all the ailments caused by B. cereus, however further research needs to be done to isolate the pharmaceutical compounds, investigate their mode of action and the effect of the same in the in vivo environment.