In vitro evaluation of antibacterial activity of infused Cola nitida seeds
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Date
2014
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Journal ISSN
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Publisher
Int.J.Curr.Microbiol.App.Sci
Abstract
The aim of the current work is to investigate the antibacterial activity of infused
Cola nitida seeds. Antibacterial screening was done using agar well diffusion
method against Bacillus cereus, Serratia marcescens, Staphyloccus epidermidis,
Proteus vulgaris and Salmonella typhi. The extract inhibited the growth of
Bacillus cereus with a zone of inhibition of 14.33±0.882, Serratia marcescens
26.67±1.202 and Staphylococcus epidermidis 12.33±0.577. The penicillin control
showed large zones of inhibition and DMSO did not show any zones of inhibition.
Analysis of variance (ANOVA) performed on the data showed that there were
significant differences in the zone of inhibition formed by the extract and antibiotic
control (p<0.0001). A Tukey
s multiple comparison tests also revealed several
differences in the zones of inhibition of the extract between organisms and between
all the organisms and their controls. Few pairwise comparisons showed that the
zones did not differ significantly between organisms. The present study has proved
that it is possible to control the growth of some pathogenic microorganisms using
the infused extract of Cola nitida. The infused extracts from the plant can therefore
be considered by pharmaceutical industries in production of cheap, affordable and
available drugs for the cure of infections caused by these organisms in situations
where expensive conventional drugs are unavailable.