Browsing by Author "Obey Jackie"

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    In Vitro control of selected pathogenic organisms by Vernonia adoensis roots
    (INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES, 2013-08-01) T. Anthoney Swamy; Obey Jackie; Ngule Chrispus Mutuku
    The main aim of conducting this study was to evaluate antibacterial activity of methanol–water extract of Vernonia adoensis roots against Salmonella typi, Klebsiella sp, Bacillus cereus, Streptococcus pyogenes, E. coli, Proteus vulgaris and Enterobacter aerogenes.The roots powder of the plant were extracted with methanol and water in the ratio 9:1. The antibacterial activity of the extract was determined by agar well diffusion method. Vernonia adeonsis roots extract was found to control the growth of only B. cereus, S. pyogenes and Proteus vulgaris at zone of inhibition of 10.50 ± 0.563, 15.67 ± 0.422 and 11.00 ± 0.577 respectively. From the study methanol- water extract can be used to control B. cereus, S. pyogenes and Proteus vulgaris. More research needs to be done to identify the specific bioactive compounds, their structural formulas, their mode of action and toxicity in vivo conditions.
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    Preliminary phytochemical and in vitro control of selected pathogenic organisms by ethanolic extract of Garcinia kola seeds
    (Int.J.Curr.Microbiol.App.Sci, 2014) Obey Jackie; T.Anthoney Swamy; Ngule Chrispus Mutuku
    Garcinia kola is cultivated for its economic importance. The plant is mainly used as a medicinal herb in West Africa. Although it is bitter the plant is used as a snack and a stimulant due to the high content of caffeine in the seeds of the plant. The current study was done to investigate the antibacterial activity of the plant seeds and to analyse the presence of important pharmaceutical compounds. From the study the plant seeds were found to contain tannins, saponins, flavonoids, terpenoids, glycosides and alkaloids but phenols and steroids were found to be absent in the plant seeds. Among all the organisms tested with the ethanol extract of G. kola, only B. cereus with an inhibition zone of 10.17±0.477 and E. coli with an inhibition zone of 12.83±0.833 were inhibited. All other organisms were not inhibited. The penicillin control showed large zones of inhibition and DMSO did not show any zones of inhibition. The data obtained in this research is a scientific justification of the plants traditional use in the treatment of various stomach problems. From this research it is worthy to recommend the plant seeds for the treatment of diarrhea caused by E.coli and all the ailments caused by B. cereus, however further research needs to be done to isolate the pharmaceutical compounds, investigate their mode of action and the effect of the same in the in vivo environment.