Browsing by Author "Ngule Chrispus Mutuku"

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    IN VITRO ANTIBACTERIAL ACTIVITY OF METHANOLIC – AQUA EXTRACT OF PLECTRANTHUS ARGENTATUS LEAVES
    (World Journal of Pharmaceutical research, 2013-11-23) Anthoney Swamy T; Jackie Obey K; Ngule Chrispus Mutuku
    The study was conducted to analyze the antibacterial activity of Plectranthus argentatus plant leaves. The plant sample was extracted using methanol and water in the ratio 9:1. From the study the plant Plectranthus argentatus was found to inhibit the growth of Escherichia coli with a zone of inhibition of 11.67 ± 0.882, Serratia liquefaciens zone of inhibition of 12.67 ± 0.667, Bacillus cereus 28.00 ± 1.154 and Proteus vulgaris 11.00 ± 0.577. The bacteria which had a zone of inhibition of less than 8 mm were termed to be unsusceptible. The data collected and documented in this paper is a scientific justification that the plant Plectranthus argentatus can be used to treat against various diseases caused by E.coli, S. liquefaciens, Bacillus cereus and Proteus vulgaris. However, more scientific data needs to be provided to indicate the mode action of the plant active compounds against the pathogens.
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    IN VITRO CONTROL OF SELECTED PATHOGENIC ORGANISMS BY METHANOLIC-AQUA EXTRACT OF ACANTHOSPERMUM AUSTRALE LEAVES
    (International Journal of Pharmaceutical Research & Analysis, 2014) Ngule Chrispus Mutuku; Jackie K Obey; Anthoney Swamy T
    The current study was done to analyse the antibacterial activity of the methanolic- aqua extract of Acanthospermum australe. From the study, the plant was found to inhibit only Bacillus cereus (20.00± 0.000) among all the other organisms the plant was tested against. Penicillin inhibited the growth of all the organisms while dimethyl sulfoxide (DMSO) did not show any zones of inhibition. The study shows that the plant can be used to treat against infections caused by Bacillus cereus, however; more research needs to be done to isolate the active compounds, determine their structural composition and their mode action in inhibiting the bacteria.
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    In Vitro control of selected pathogenic organisms by Vernonia adoensis roots
    (INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES, 2013-08-01) T. Anthoney Swamy; Obey Jackie; Ngule Chrispus Mutuku
    The main aim of conducting this study was to evaluate antibacterial activity of methanol–water extract of Vernonia adoensis roots against Salmonella typi, Klebsiella sp, Bacillus cereus, Streptococcus pyogenes, E. coli, Proteus vulgaris and Enterobacter aerogenes.The roots powder of the plant were extracted with methanol and water in the ratio 9:1. The antibacterial activity of the extract was determined by agar well diffusion method. Vernonia adeonsis roots extract was found to control the growth of only B. cereus, S. pyogenes and Proteus vulgaris at zone of inhibition of 10.50 ± 0.563, 15.67 ± 0.422 and 11.00 ± 0.577 respectively. From the study methanol- water extract can be used to control B. cereus, S. pyogenes and Proteus vulgaris. More research needs to be done to identify the specific bioactive compounds, their structural formulas, their mode of action and toxicity in vivo conditions.
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    Preliminary phytochemical and in vitro control of selected pathogenic organisms by ethanolic extract of Garcinia kola seeds
    (Int.J.Curr.Microbiol.App.Sci, 2014) Obey Jackie; T.Anthoney Swamy; Ngule Chrispus Mutuku
    Garcinia kola is cultivated for its economic importance. The plant is mainly used as a medicinal herb in West Africa. Although it is bitter the plant is used as a snack and a stimulant due to the high content of caffeine in the seeds of the plant. The current study was done to investigate the antibacterial activity of the plant seeds and to analyse the presence of important pharmaceutical compounds. From the study the plant seeds were found to contain tannins, saponins, flavonoids, terpenoids, glycosides and alkaloids but phenols and steroids were found to be absent in the plant seeds. Among all the organisms tested with the ethanol extract of G. kola, only B. cereus with an inhibition zone of 10.17±0.477 and E. coli with an inhibition zone of 12.83±0.833 were inhibited. All other organisms were not inhibited. The penicillin control showed large zones of inhibition and DMSO did not show any zones of inhibition. The data obtained in this research is a scientific justification of the plants traditional use in the treatment of various stomach problems. From this research it is worthy to recommend the plant seeds for the treatment of diarrhea caused by E.coli and all the ailments caused by B. cereus, however further research needs to be done to isolate the pharmaceutical compounds, investigate their mode of action and the effect of the same in the in vivo environment.
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    QUALITATIVE ANALYSIS OF PHYTOCONSTITUENTS IN TRAGIA BREVIPES PLANT
    (International Journal of Pharmaceutical Research & Analysis, 2013) T Anthoney Swamy1; Ngule Chrispus Mutuku; Makau Elijah Ngule; Ramesh Francis
    The study was conducted to analyze the phytoconstituents of Tragia brevipes plant leaves. The plant samples were extracted using methanol and water in the ratio 9:1. The phytoconstituents study was done using standard procedures [18, 19 and 20]. From the study Tragia brevipes was found to contain tannins, saponins, terpenoids, flavonoids, cardiac glycoside, phenols, steroidal nucleus and steroidal ring, but alkaloids and steroids were found to be absent in the plant extract.