Browsing by Author "Miyogo Edwin"

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    In Vitro Antibacterial activity of Ethanolic - aqua extract of Tagetes minuta leaves harvested from The University of Eastern Africa, Baraton, Nandi County, Kenya
    (INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES, 2015-05) Anthoney Swamy T; Jackie K. Obey; Terer E. Kipngetich; Miyogo Edwin
    Tagetes minuta is a plant that has been used in traditional medicine for the treatment of various illnesses. In this study, the ethanolic extract of T. minuta was tested for its antibacterial activity against selected microorganisms of clinical significance. In an antibiotic susceptibility assay using the plant extract and DMSO control, the results from the study showed that the extract was active against Proteus vulgaris with zone of inhibition of 17.30±0.333mm, Escherichia coli 9.30±0.333mm, Salmonella typhi 11.83±0.44mm, Bacillus cereus 8.00±0.000 and Enterobacter aerogenes 16.67±0.882mm. The penicillin positive control showed high zones of inhibition while the DMSO negative control showed no zones of inhibition. An analysis of variance test on the results showed that there were significant differences in the zones of inhibition of the extract and penicillin against all the organisms (p<0.0001). These results have shown that the growth of these organisms can be controlled with the extract, hence making the extract components potential agents for incorporation into drug production.
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    IN VITRO ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF PITTOSPORUM VIRIDIFLORUM LEAVES EXTRACT AGAINST LABORATORY STRAINS OF SELECTED MICROORGANISMS
    (International Journal of Bioassays, 2014-08-08) Anthoney Swamy T; Jackie K Obey; Terer Erick Kipngetich; Miyogo Edwin
    The aim of this study was to analyse the antibacterial Activity of Pittosporum viridiflorum leaves extract against laboratory strains of selected microorganisms. Infusions of the bark of P. viridiflorum are used to treat stomach complaints, chest pain, malaria and other fever. The mean zones of inhibition of the extract against microorganisms were 12.67±0.882 mm for Enterobacter aerogenes, 12.50±0.281mm for Escherichia coli, 11.67±0.333 mm for Proteus vulgaris, 11.67±0.000mm for Bacillus cereus and 7.67±0.333mm for Salmonella typhi. The penicillin positive control showed large zones of inhibition and the dimethylsulfoxide negative control did not show any zone of inhibition. This report suggests that the extract was active against all the other organisms except S. typhi because the zone of inhibitions was less than 8 mm. Analysis of variance showed that the zones of inhibition of the extract and antibiotic control against the microorganisms were significantly different (p<0.0001). The Tukey’s honestly test further showed both significant and non-significant comparisons between the extract and controls for various bacterial organisms. This study has shown that controlling the growth of microorganisms in vitro can be achieved by the ethanolic extract of P. viridiflorum.