Browsing by Author "Willy K Kemboi"

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    HORIZONTAL GENE TRANSFER OF DRUG RESISTANCE GENES BETWEEN SALMONELLA AND ESCHERICHIA COLI
    (International Journal of Bioassays, 2014-03-12) Willy K Kemboi; Wanje Raphael; Ramesh F
    The physiological process that takes in the living organism is coded by a section of the DNA, the gene. Even resistance to antimicrobial by bacteria is conferred by resistant gene. Resistant Salmonella spp. and susceptible E. coli was used to study drug resistance gene transfer, which can be transferred by conjugation, transduction, or transformation. Materials used are, DST media, inoculating loops and sterile swaps Furthermore, eight antibiotics were used in the study. The susceptibility and resistance of the E, coli and Salmonella spp. was first confirmed. Diagnostic sensitivity test of the E. coli under three conditions (E. coli+ Dead Salmonella spp, E. coli+ live Salmonella spp, E. coli+ DNA) were done. Results from the study confirm that the susceptible bacteria, E. coli was able to acquire resistance genes from resistant Salmonella spp under the three conditions. Results proved that the most efficient means of acquiring resistance genes is through conjugation. It is recommended antibiotics that alter the cell wall as the most effective in capping resistance.
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    USING CONVENTIONAL METHODS TO DETERMINE THE LEVEL OF TOILET SEAT CONTAMINATION BY TRICHOMONAS VAGINALIS IN THE UNIVERSITY OF EASTERN AFRICA, BARATON, KENYA
    (International Journal of Bioassays, 2015-01-14) Willy K Kemboi; Jackie Grear; Ramesh F
    This study pursued to investigate the possibility of transmission of sexually transmitted disease Trichomoniasis, via a toilet seat. The disease is caused by a single cell protozoan Trichomonas vaginalis. For quite some time, acquisition of Trichomoniasis is believed to be through sexual intercourse and little evidence is via sharing the bathroom tools. A number of investigations have been done and published on the possible relationship that infection with Trichomoniasis escalates the infected susceptibility to HIV/AIDS. In this study, women toilets seats were examined in University of Eastern Africa, Baraton using; wet mount and Gram stain method. The data collected and examined showed that there is statistically significant evidence that trophozoites deposited by infected women on the toilet seats in University of Eastern Africa, Baraton, can cause an infection to health users (P <0.05).