Browsing by Author "Ramesh Francis"

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    Effect of Culcasia falcifolia on the biogenic amine levels in the brain tissue of pentylenetetrazole induced seizure in mice
    (GSC Biological and Pharmaceutical Sciences, 2019-04) Gracelyn Portia Anthony Doss; Ramesh Francis
    In the present study the effect of the ethanol extract of Culcasia falcifolia on the neurotransmitter levels were evaluated. Pentylenetetrazole (PTZ) (60 mg/kg body weight, i. p) was administered to all the groups of mice to induce convulsions. After observing the convulsion, all groups rats were sacrificed, whole brain was dissected out and separated the forebrain. The brain tissue was homogenized and then centrifuged. The aqueous phase was taken for the assay. The reading was obtained using spectroflurimeter. The result of the present study shows that the ethanol extract of Culcasia falcifolia at the dose of 200 and 400 mg/kg body weight significantly increased the level of inhibitory neurotransmitter GABA and also showed significant increase in the levels of dopamine, noradrenalin and serotonin when compared to seizure control group (PTZ treated group) and was almost equivalent to control group. The findings of the study showed that the extract at 200 and 400 mg/kg body weight significantly (p<0.001) increased the levels of GABA, dopamine, serotonin and noradrenaline. The ethanol extract of Culcasia falcifolia exerted antiepileptic activity by decreasing seizure susceptibility to pentylenetetrazole-induced seizure in mice due to restoration of GABA, serotonin, dopamine and noradrenaline concentration levels in mice brain.
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    EVALUATION OF DNA DAMAGE OF THE FISH CLARIAS BATRACHUS EXPOSED TO SAGO EFFLUE T USI G THE COMET ASSAY
    (European International Journal of Science and Technology, 2023-04) Ramesh Francis; K. agarajan*
    In this study, the D A damages were observed by comet assay carried out in the blood samples of the fish Clariasbatrachus exposed to control and different concentrations of treated Sago effluent. The concentration chosen were 25%, 50% and 100%.The D A damage was determined by the length of comet tail. The control fish showed normal structure whereas the effluent exposed individuals registered alterations in the comet tail. The length of the comet tail was increased with increasing the concentration of the effluent.
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    Impact of haematological characteristic alteration in sago factory effluent treated fish Clasrias batrachus
    (GSC Biological and Pharmaceutical Sciences, 2019-05-14) Ramesh Francis
    In the present study was undertaken to evaluate the Sago effluent toxicity stress symptoms in the fish blood during a long term period. The effect of sago effluent on various Haematological parameters were evaluated exposing the fresh water fish Clarias batrachus to different concentration i.e., (Control, 25%, 50% and 75% Sago effluent concentrations respectively. Changes were observed after 28 days of exposure, (sublethal concentration). Red Blood Cells count (RBC), Haematocrit value (HCT), Mean Corpuscular Volume (MCV) and Mean Corpuscular Haemoglobin (MCH) content decreased when compared to the control. The number of White Blood Cells count (WBC) increased in the effluent treated fishes. Increase and decrease in haematological indices means that fish exposed to effluents were under stress.
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    QUALITATIVE ANALYSIS OF PHYTOCONSTITUENTS IN TRAGIA BREVIPES PLANT
    (International Journal of Pharmaceutical Research & Analysis, 2013) T Anthoney Swamy1; Ngule Chrispus Mutuku; Makau Elijah Ngule; Ramesh Francis
    The study was conducted to analyze the phytoconstituents of Tragia brevipes plant leaves. The plant samples were extracted using methanol and water in the ratio 9:1. The phytoconstituents study was done using standard procedures [18, 19 and 20]. From the study Tragia brevipes was found to contain tannins, saponins, terpenoids, flavonoids, cardiac glycoside, phenols, steroidal nucleus and steroidal ring, but alkaloids and steroids were found to be absent in the plant extract.