Browsing by Author "Obey Jackie K"

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    IN VITRO ANTIBACTERIAL ACTIVITY OF THE AQUA EXTRACT OF PHYTOLACCA DODECANDRA ROOTS AGAINST LABORATORY STRAINS OF SELECTED HUMAN PATHOGENIC ORGANISMS
    (INTERNATIONAL JOURNAL OF BIOASSAYS, 2015-04-22) Anthoney Swamy T; Obey Jackie K; Miyogo Edwin O; Terer Erick
    The aim of this study is to test the antibacterial activity of the aqua extract of Phytolacca dodecandra roots against Bacillus cereus, Staphylococcus epidermidis, Streptococcus pyogenes, Escherichia coli, Serratia marcescens, Serratia liquefaciens, Salmonella typhi and Enterobacter aerogenes. The agar well diffusion method was used to determine the zones of inhibition by the extract, penicillin antibiotic (positive control) and sterile distilled water (negative control) against the organisms. The extract showed the highest activity against Enterobacter aerogenes (16.333+0.882 mm), followed by Bacillus cereus (14.33±0.333 mm), Escherichia coli (14.00±0.000 mm), Streptococcus pyogenes (13.00±0.000 mm). The zone of inhibition against Serratia marcescens was 7.33±0.333 mm, considered inactive because it is less than 8 mm. All the other organisms were not inhibited by the extract. The Tukey’s significant difference test showed significant differences with most of the pair-wise comparisons while a few zones were not significantly different from the other. The results from this study have shown that the aqua extract of P. dodecandra roots can inhibit both gram negative and gram positive bacteria in vitro. The pharmacologic mechanisms of action of the active ingredients must be studied and the active components can be considered for possible incorporation into conventional antibacterial drug production.
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    PHYTOPHARMACOLOGICAL ANALYSIS OF METHANOLIC-AQUA EXTRACT (FRACTIONS) OF SENNA DIDYMOBOTRYA ROOTS
    (2023-09) Anthoney Swamy Thangiah; Mutuku Chrispus Ngule; Obey Jackie K
    The study was carried to analyze the phytochemical constituents of the Senna didymobotrya roots. The plant samples were extracted using methanol and water in the ratio of 9:1. The crude extract was disintegrated using different solvents according to their polarity. The solvents used were chloroform, ethyl acetate, butanol and water respectively. All the extracts were subjected to phytochemical study using standard procedures. From the study the of crude extract of Senna didymobotrya was found to contain tannins, saponins, terpenoids, flavonoids, phenols, alkaloids, steroidal rings but steroids were found to be absent. The presence of these important phytochemicals in the plant roots is a scientific justification of the use of the plant in the traditional treatment against various diseases affecting humans and animals. However, more research needs to be done to identify the specific compounds, there structural formulas and their medicinal values.
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    PRELIMINARY PHYTOCHEMICAL SCREENING OF METHANOLIC–AQUA EXTRACT OF ACANTHOSPERMUM AUSTRALE LEAVES
    (2013-09-11) AUSTRALE LEAVES Anthoney Swamy Thangiah; Mutuku Chrispus Ngule; Obey Jackie K
    The study was carried to analyze the phytochemical constituents of the Acanthospermum australe. The plant samples were extracted using methanol and water in the ratio of 9:1. From the study the extract of Acanthospermum australe was found to contain tannins, saponins, terpenoids, phenols, alkaloids, steroidal rings but steroids and flavonoids were found to be absent. The presence of these important phytochemicals in the plant roots is a scientific justification of the plant use in the traditional treatment against various diseases affecting humans and animals. However, more research needs to be done to identify the specific compounds, their structural formulas and contribution towards medicinal values of the plant.