Browsing by Author "Mueni Hellen Ndiku"

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    Antibacterial activity and Preliminary phytochemical screening of hot- aqua extract of Tetradenia riparia leaves
    (INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES, 2014-08) Mueni Hellen Ndiku; Mutuku Chrispus Ngule
    Nature is a paradise of medicinal solutions to all ailments affecting human beings. Medicinal plants have been used widely to treat against the currently widespread strains of drug resistant bacteria. Green medicine has attracted great interest due to the belief that it is safe, cheap and more dependable than aliphatic drugs, which have adverse side effects. The current study was done to analyze the Phytochemical and antibacterial activity of hot – aqua extract of Tetradenia riparia leaves. The Phytochemical results showed the plant to contain very important compounds (Table 1). The infused plant extract (Table 2) inhibited the growth of all the microorganisms tested. Bacillus cereus was the microorganism which had the highest zone of inhibition of 22.67± 0.453, followed by Serratia liquefaciens with an inhibition zone of 21.00±0.577, Enterobacter aerogenes inhibition zone of 14.33±0.333, Salmonella typhi, 13.33±0.333 and Escherichia coli, 13.33±0.887. Penicillin which was used as the positive control inhibited all the microorganisms while dimethyl sulfoxide (DMSO) which was used was used as the negative control did not show any zones of inhibition against the microorganisms used in the study. The results obtained in this study shows that the plant’s potency in the treatment against all the bacterial organisms tested. However further research needs to be done to isolate the active compounds, determine their structure, their mode of action and their effect in the in vivo environment.
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    PRELIMINARY PHYTOCHEMICAL AND ANTIBACTERIAL SCREENING OF FRESH TETRADENIA RIPARIA LEAVES WATER EXTRACT AGAINST SELECTED PATHOGENIC MICROORGANISMS
    (International Journal of Bioassays, 2014-05-17) Mutuku Chrispus Ngule; Mueni Hellen Ndiku; F Ramesh; Makau Elijah Ngule
    The use of plants as a source of medicine is as old as man himself. Plants have been used since time immemorial to treat against various diseases affecting human beings all over the world. Before the invention of synthetic drugs, traditional medicine dominated the world. The study was conducted to analyze the antibacterial activity phytoconstituents of Tetradenia riparia plant. The samples were extracted using water solvent. The phytoconstituents study was done using standard procedures. The bioassay was done using well diffusion method. From the study, Tetradenia riparia was found to contain tannins, saponins, flavonoids, cardiac glycosides, phenols, alkaloids and steroidal rings, but terpenoids, steroids and steroidal nucleus were found to be absent in the plant extract. The fresh leaves water extract of the plant Tetradenia riparia inhibited the growth of all the microorganisms used. The zones of inhibition were high in Salmonella typhi (21.00±0.000), followed by Staphylococcus epidermidis, Proteus vulgaris and Bacillus cereus with inhibition zones of 18.67±0.577, 18.00±0.333 and 18.00±0.577 respectively. Serratia marcescens and Serratia liquefaciens had inhibition zones of 13.00±0.333 and 13.33±0.577 respectively. Escherichia coli had the least inhibition zone of 9.67±0.577. Penicillin which was used as the positive control inhibited the growth of all the microorganisms tested while DMSO (negative control) did not show any inhibition zones against any of the microorganisms tested. The current study may be a partial scientific justification of the plants use in the treatment against the infections caused by all the tested microorganisms.